CEInfinite: icIEF-MS Online
Seamlessly coupling icIEF-MS with your mass spectrometer for rapid intact mass analysis and critical quality attribute characterization.
Rapid Characterization of CQAs and Emerging Modalities
CEInfinite couples directly to your preferred mass spectrometer for the rapid characterization of multiple product attributes. The addition of AES consumables enables outstanding reproducibility and sensitivity.
icIEF-MS enables rapid feedback on the post-translational modifications (PTMs) and other critical quality attributes (CQAs) underlying each protein charge isomer.
This can inform decision making during process development, and help mitigate unexpected setbacks during regulatory filing, scale-up, and manufacturing. Furthermore, it can help characterize ADCs, LNPs, another emerging modality that are challenging to characterize using other techniques.
With the introduction of icIEF-MS on the CEInfinite you can couple to a variety of mass spectrometers including the Orbitrap-based instruments for rapid intact mass analysis.
How Does icIEF-MS Coupling Work?
icIEF fractionation setup was converted into an icIEF-MS system by directly coupling the transfer capillary to a mass spectrometer. This enables intact mass analysis in 45 minutes or less for more rapid feedback on icIEF peaks.
By utilizing cartridges of 200 µm diameter, the resolution is prioritized, and importantly, acetonitrile and formic acid are added to the makeup solution to help prepare the icIEF peaks for electrospray ionization.
Popular Capabilities for icIEF-MS on CEInfinite
MS Capabilities
MS Agnostic
Couple to Thermo Fisher, Waters, Agilent, Bruker, etc.
Fast, Easy Connectivity
Option to connect to nanospray interface in 5 min.
Chromeleon Driver
icIEF and Thermo Fisher MS control with single software.
Versatility
High Throughput
30-45 min/run for rapid intact mass analysis.
Native MS for ADCs
Flexibility with the makeup solution composition.
Reproducibility
Pressure mobilization and durable capillary coatings.
Consumables
MS Compatibility
Remove MC from solution with CEInfinite coatings.
AESlyte®
Improved resolution and minimized MS background.
Cartridge Lifetime
50+ runs for most samples with no upper limit.
icIEF-MS Under Native Condition
Native mass spectrometry (nMS) is an emerging approach for studying large biomolecules and their complexes in the solution via the electrospray ionization mass spectrometry (ESI-MS), while maintaining as much as possible the native structural features of the analytes and their interactions in the gas phase during the electrospray. In biopharmaceutical development, native MS is a versatile method used to study complex protein heterogeneity with non-covalently driven assemblies in a native-like folded state.
AES develops a robust imaged capillary isoelectric focusing (icIEF) coupled to MS workflow under native condition, to characterize cysteine-linked antibody-drug-conjugate (ADC) charge heterogeneity. Successful separation and reliable identification of charge variants of latest-generation cysteine-linked ADCs including polatuzumab vedotin and brentuximab vedotin were achieved using icIEF-MS online platform under native condition. The developed icIEF-MS under native condition can be applied to the diverse complex proteins that are unstable under denaturing condition.
Popular Applications for icIEF-MS on CEInfinite
Plan for success with in-depth information throughout all stages of biopharmaceutical development and manufacturing. Leverage a single icIEF-MS platform for rapid and comprehensive identification of several critical quality attributes (CQAs).
Early-Stage Development
High-throughput intact mass analysis of icIEF peaks. Understand potential post-translation modifications (PTMs) and product variations from the outset. Improve decision-making with rapid feedback on multiple product attributes. Optimize cell line development, process development, and more.
Manufacturing
Evaluate the potential of icIEF-MS to help improve batch consistency for drug safety and efficacy. Reduce costs by monitoring several CQAs simultaneously on a single platform. Enable immediate identification of new and unexpected icIEF peaks to help promptly resolve production issues.
Instrument Spare Parts
Technical Notes & Publications
Chromeleon - CEInfinite
AES_TN_Advanced icIEF Analysis
CEInsight CM7 Driver MS Setup Manual
CEInsight CM7 Driver Operation Manual
Update on Rapid Progress of icIEF
Faster. More Accurate. icIEF-MS Simplified.
Achieve high-resolution intact mass analysis with seamless icIEF-MS integration. Get better data, faster—without the bottlenecks. Let’s discuss how CEInfinite can elevate your workflow.
