Resources

Recent developments of imaged capillary isoelectric focusing technology for in-depth biopharmaceutical characterization

Advancing protein heterogeneity analysis: Nano-flow pressure mobilization for precise icIEF fractionation and online MS detection

An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: High-efficient fractionation and online mass spectrometry detection

An Imaged Capillary Isoelectric Focusing Separation of the Linear and Cyclic Variants of a Mimotope of the Cancer-Related CD20 Antigen–Validation and Statistical Evaluation

In-depth characterization of a cysteine-linked ADC disitamab vedotin by multiple LC-MS analysis methods and cutting-edge imaged capillary isoelectric focusing coupled with native mass spectrometry

Imaged Capillary Isoelectric Focusing Coupled to High-Resolution Mass Spectrometry (icIEF-MS) for Cysteine-Linked Antibody–Drug Conjugate (ADC) Heterogeneity Characterization Under Native Condition

Mass spectrometry study on SARS-CoV-2 recombinant vaccine with comprehensive separation techniques to characterize complex heterogeneity

Imaged capillary isoelectric focusing tandem high-resolution mass spectrometry using nano electrospray ionization (ESI) for protein heterogeneity characterization

Imaged capillary isoelectric focusing employing fluorocarbon and methylcellulose coated fused silica capillary for characterization of charge heterogeneity of protein biopharmaceuticals

Integrating ultra-high-performance liquid chromatography tandem mass spectrometry and imaged capillary isoelectric focusing for in-depth characterization of complex fusion proteins

Fractionation and online mass spectrometry based on imaged capillary isoelectric focusing (icIEF) for characterizing charge heterogeneity of therapeutic antibody

Mass Spectrometry-Based Charge Heterogeneity Characterization of Therapeutic mAbs with Imaged Capillary Isoelectric Focusing and Ion-Exchange Chromatography as Separation Techniques

High-efficient characterization of complex protein drugs by imaged capillary isoelectric focusing

Imaged capillary isoelectric focusing (icIEF) tandem high resolution mass spectrometry for charged heterogeneity of protein drugs in biopharmaceutical discovery

Cutting-edge mass spectrometry strategy based on imaged capillary isoelectric focusing (icIEF) technology for characterizing charge heterogeneity of monoclonal antibody

White paper: Critical reagents for successful iCIEF

Characterization of Therapeutic mAb Charge Heterogeneity by iCIEF Coupled to Mass Spectrometry (iCIEF–MS)Global characterization of modifications to the charge isomers of IgG antibody

Global characterization of modifications to the charge isomers of IgG antibody

High-Efficiency Preparative Imaged Capillary Isoelectric Focusing (iCIEF) and iCIEF-MS Protein Charge Variant Characterisation

High-Throughput Precision with On-Board Mixing for Imaged Capillary Isoelectric Focusing (iCIEF)

Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve

Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging

• CEInfinite Tech Note – AESlyte® Carrier Ampholytes
• Application Note – Acidic Peak Focus Fractionation Collection
• Application Note – icIEF-HRMS
• Tech Note – MC Coating Cartridge

Imaged capillary isoelectric focusing(iCIEF) coupled to high resolution mass spectrometry sharpens depth and width in biopharmaceutical discovery

Teresa Kwok, Xiaoxi Zhang, She Lin Chan, Zhen Long, Jessica Shi, Mike Zhou, Min Du, Tony Chen, Victor Li, Tao Bo, Tiemin Huang

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Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform

Xiaoxi Zhang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du

https://assets.thermofisher.cn/TFS-Assets/CMD/posters/po-66139-asms22-monoclonal-antibodies-antibody-drug-conjugates-po66139-en.pdf, 2022

In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis

Xiaoxi Zhang, Zhigui Zhou, Min Sang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du

https://assets.thermofisher.cn/TFS-Assets/CMD/posters/sub163-PO66105-ASMS2021-iCIEF-MS-Xiaoxi-v2-DFbrand-cbk-final.pdf, 2022

The Expanding Hyphenation of LC Techniques to MS for Intact Mass Characterization of Biopharmaceuticals During Development – Lessons Learned and The Road Ahead

Dan Bach Kristensen, Symphogen

17th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry, September 2021

(Application Note) Comparison of Different Brands of Carrier Ampholytes for Monoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing

Christian Wenz, Agilent Technologies, Inc.

Biotherapeutics & Biosimilars, www.agilent.com/chem, 2017, Download PDF