Posters
NEW Imaged capillary isoelectric focusing(iCIEF) coupled to high resolution mass spectrometry sharpens depth and width in biopharmaceutical discovery
Teresa Kwok, Xiaoxi Zhang, She Lin Chan, Zhen Long, Jessica Shi, Mike Zhou, Min Du, Tony Chen, Victor Li, Tao Bo, Tiemin Huang
Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform
Xiaoxi Zhang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du
https://assets.thermofisher.cn/TFS-Assets/CMD/posters/po-66139-asms22-monoclonal-antibodies-antibody-drug-conjugates-po66139-en.pdf, 2022
In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis
Xiaoxi Zhang, Zhigui Zhou, Min Sang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du
https://assets.thermofisher.cn/TFS-Assets/CMD/posters/sub163-PO66105-ASMS2021-iCIEF-MS-Xiaoxi-v2-DFbrand-cbk-final.pdf, 2022
Publications
NEW Mass spectrometry study on SARS-CoV-2 recombinant vaccine with comprehensive separation techniques to characterize complex heterogeneity
Gang Wu, Jialiang Du, Chuanfei Yu, Zhihao Fu, Xiaoxi Zhang, Lan Wang, Junzhi Wang
Analytica Chimica Acta, Available online 12 February 2024, 342349; https://doi.org/10.1016/j.aca.2024.342349
Imaged capillary isoelectric focusing tandem high-resolution mass spectrometry using nano electrospray ionization (ESI) for protein heterogeneity characterization
Teresa Kwok, She Lin Chan, Matthew Courtney, Mike Zhou, Tiemin Huang, Tao Bo, Victor Li, Tong Chen
Analytical Biochemistry, Volume 680, 1 November 2023, 115312; https://doi.org/10.1016/j.ab.2023.115312
Imaged capillary isoelectric focusing employing fluorocarbon and methylcellulose coated fused silica capillary for characterization of charge heterogeneity of protein biopharmaceuticals
Teresa Kwok, She Lin Chan, Jessica Shi, Mike Zhou, Anna Schaefer, Tao Bo, Victor Li, Tiemin Huang, Tong Chen
Separation Science Plus, 2023;2200160; https://doi.org/10.1002/sscp.202200160
Integrating ultra-high-performance liquid chromatography tandem mass spectrometry and imaged capillary isoelectric focusing for in-depth characterization of complex fusion proteins
Wenhong Fan, Xiang Li, Zhen Long, Dening Pei, Xinchang Shi, Guangyu Wang, Ying Guo, Tao Bo, Yong Zhou, and Tong Chen
Rapid Commun Mass Spectrom, 2023;37(8):e9484. doi:10.1002/rcm. 9484
Fractionation and online mass spectrometry based on imaged capillary isoelectric focusing (icIEF) for characterizing charge heterogeneity of therapeutic antibody
Teresa Kwok, Mike Zhou, Anna Schaefer, Tao Bo, Victor Li, Tiemin Huang and Tong Chen
Analytical Methods, Volume 15 (2023)
Mass Spectrometry-Based Charge Heterogeneity Characterization of Therapeutic mAbs with Imaged Capillary Isoelectric Focusing and Ion-Exchange Chromatography as Separation Techniques
Gang Wu, Chuanfei Yu, Wenbo Wang, Jialiang Du, Zhihao Fu, Gangling Xu, Meng Li, and Lan Wang
Analytical Chemistry, Volume 95 (2023)，https://doi.org/10.1021/acs.analchem.2c05071
High-efficient characterization of complex protein drugs by imaged capillary isoelectric focusing
Teresa Kwok, She Lin Chan, Mike Zhou, Anna Schaefer, Tao Bo, Tiemin Huang, Victor Li, Tong Chen
Separation Sciences Plus (2022), https://doi.org/10.1002/sscp.202200142
Imaged capillary isoelectric focusing (icIEF) tandem high resolution mass spectrometry for charged heterogeneity of protein drugs in biopharmaceutical discovery
Xiaoxi Zhang, Teresa Kwok, Mike Zhou, Min Du, Victor Li, Tao Bo, Tiemin Huang, Tong Chen
Journal of Pharmaceutical and Biomedical Analysis 224 (2023) 115178, https://doi.org/10.1016/j.jpba.2022.115178
Cutting-edge mass spectrometry strategy based on imaged capillary isoelectric focusing (icIEF) technology for characterizing charge heterogeneity of monoclonal antibody
Xiaoxi Zhang, Tong Chen, Victor Li, Tao Bo, Min Du, Tiemin Huang
Analytical Biochemistry, Volume 660, 1 January 2023, 114961
White paper: Critical reagents for successful iCIEF
Advanced Electrophoresis Solutions Ltd., October, 2022
Characterization of Therapeutic mAb Charge Heterogeneity by iCIEF Coupled to Mass Spectrometry (iCIEF–MS)Global characterization of modifications to the charge isomers of IgG antibody
Johannes Schlecht, Bernd Moritz, Steffen Kiessig, Christian Neusüß
September23, 2022, https://orcid.org/0000-0001-9071-5904
Global characterization of modifications to the charge isomers of IgG antibody
Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao,Xiaohong Qian, Tao Zhu, Wantao Ying
Journal of Pharmaceutical Analysis, 12 (2022) 156-163
High-Efficiency Preparative Imaged Capillary Isoelectric Focusing (iCIEF) and iCIEF-MS Protein Charge Variant Characterisation
Tong Chen, Teresa Kwok, Anne Rose Esmin, Victor Li, Gerard Rozing,Tiemin Huang
Chromatography, May / June 2021
High-Throughput Precision with On-Board Mixing for Imaged Capillary Isoelectric Focusing (iCIEF)
Katelyn Cousteils, Jennifer Hu, Victor Li, Tiemin Huang
Chromatography, February / March 2019
Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve
Faculty of Chemistry, Aalen University, Aalen, Germany
Electrophoresis, 2018, DOI 10.1002/elps.201800013
Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging
Junjian Fang*, Jiexin Liu*, Haijing Li, Jianhua Cheng, Shengming Wu, and Fangting Dong
INSTRUMENTATION SCIENCE AND TECHNOLOGY, 2018, https://doi.org/10.1080/10739149.2017.1405821
Others
The Expanding Hyphenation of LC Techniques to MS for Intact Mass Characterization of Biopharmaceuticals During Development – Lessons Learned and The Road Ahead
Dan Bach Kristensen, Symphogen
17th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry, September 2021
(Application Note) Comparison of Different Brands of Carrier Ampholytes for Monoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing
Christian Wenz, Agilent Technologies, Inc.
Biotherapeutics & Biosimilars, www.agilent.com/chem, 2017