Posters

NEW  Imaged capillary isoelectric focusing(iCIEF) coupled to high resolution mass spectrometry sharpens depth and width in biopharmaceutical discovery
Teresa Kwok, Xiaoxi Zhang, She Lin Chan, Zhen Long, Jessica Shi, Mike Zhou, Min Du, Tony Chen, Victor Li, Tao Bo, Tiemin Huang

Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform
Xiaoxi Zhang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du
https://assets.thermofisher.cn/TFS-Assets/CMD/posters/po-66139-asms22-monoclonal-antibodies-antibody-drug-conjugates-po66139-en.pdf, 2022

In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis
Xiaoxi Zhang, Zhigui Zhou, Min Sang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du
https://assets.thermofisher.cn/TFS-Assets/CMD/posters/sub163-PO66105-ASMS2021-iCIEF-MS-Xiaoxi-v2-DFbrand-cbk-final.pdf, 2022

Publications

NEW Fractionation and online mass spectrometry based on imaged capillary isoelectric focusing (icIEF) for characterizing charge heterogeneity of therapeutic antibody
Teresa Kwok, Mike Zhou, Anna Schaefer, Tao Bo, Victor Li, Tiemin Huang and Tong Chen
Analytical Methods, Volume 15 (2023)

NEW Mass Spectrometry-Based Charge Heterogeneity Characterization of Therapeutic mAbs with Imaged Capillary Isoelectric Focusing and Ion-Exchange Chromatography as Separation Techniques
Gang Wu, Chuanfei Yu, Wenbo Wang, Jialiang Du, Zhihao Fu, Gangling Xu, Meng Li, and Lan Wang 
Analytical Chemistry, Volume 95 (2023) https://doi.org/10.1021/acs.analchem.2c05071

High-efficient characterization of complex protein drugs by imaged capillary isoelectric focusing
Teresa Kwok, She Lin Chan, Mike Zhou, Anna Schaefer, Tao Bo, Tiemin Huang, Victor Li, Tong Chen
Separation Sciences Plus (2022), https://doi.org/10.1002/sscp.202200142

Imaged capillary isoelectric focusing (icIEF) tandem high resolution mass spectrometry for charged heterogeneity of protein drugs in biopharmaceutical discovery
Xiaoxi Zhang, Teresa Kwok, Mike Zhou, Min Du,  Victor Li, Tao Bo, Tiemin Huang, Tong Chen
Journal of Pharmaceutical and Biomedical Analysis 224 (2023) 115178, https://doi.org/10.1016/j.jpba.2022.115178

Cutting-edge mass spectrometry strategy based on imaged capillary isoelectric focusing (icIEF) technology for characterizing charge heterogeneity of monoclonal antibody
Xiaoxi Zhang, Tong Chen, Victor Li, Tao Bo, Min Du, Tiemin Huang
Analytical Biochemistry, Volume 660, 1 January 2023, 114961

White paper: Critical reagents for successful iCIEF
Advanced Electrophoresis Solutions Ltd., October, 2022

Characterization of Therapeutic mAb Charge Heterogeneity by iCIEF Coupled to Mass Spectrometry (iCIEF–MS)Global characterization of modifications to the charge isomers of IgG antibody
Johannes Schlecht, Bernd Moritz, Steffen Kiessig, Christian Neusüß
September23, 2022, https://orcid.org/0000-0001-9071-5904

Global characterization of modifications to the charge isomers of IgG antibody
Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao,Xiaohong Qian, Tao Zhu, Wantao Ying
Journal of Pharmaceutical Analysis, 12 (2022) 156-163

High-Efficiency Preparative Imaged Capillary Isoelectric Focusing (iCIEF) and iCIEF-MS Protein Charge Variant Characterisation
Tong Chen, Teresa Kwok, Anne Rose Esmin, Victor Li, Gerard Rozing,Tiemin Huang
Chromatography, May / June 2021

High-Throughput Precision with On-Board Mixing for Imaged Capillary Isoelectric Focusing (iCIEF)
Katelyn Cousteils, Jennifer Hu, Victor Li, Tiemin Huang
Chromatography, February / March 2019

Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve
Faculty of Chemistry, Aalen University, Aalen, Germany
Electrophoresis, 2018, DOI 10.1002/elps.201800013

Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging
Junjian Fang*, Jiexin Liu*, Haijing Li, Jianhua Cheng, Shengming Wu, and Fangting Dong
INSTRUMENTATION SCIENCE AND TECHNOLOGY, 2018, https://doi.org/10.1080/10739149.2017.1405821

Others

The Expanding Hyphenation of LC Techniques to MS for Intact Mass Characterization of Biopharmaceuticals During Development – Lessons Learned and The Road Ahead
Dan Bach Kristensen, Symphogen
17th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry, September 2021