Posters

NEW  Imaged capillary isoelectric focusing(iCIEF) coupled to high resolution mass spectrometry sharpens depth and width in biopharmaceutical discovery
Teresa Kwok, Xiaoxi Zhang, She Lin Chan, Zhen Long, Jessica Shi, Mike Zhou, Min Du, Tony Chen, Victor Li, Tao Bo, Tiemin Huang

Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform
Xiaoxi Zhang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du
https://assets.thermofisher.cn/TFS-Assets/CMD/posters/po-66139-asms22-monoclonal-antibodies-antibody-drug-conjugates-po66139-en.pdf, 2022

In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis
Xiaoxi Zhang, Zhigui Zhou, Min Sang, Tiemin Huang, Tony Chen, Tao Bo, and Min Du
https://assets.thermofisher.cn/TFS-Assets/CMD/posters/sub163-PO66105-ASMS2021-iCIEF-MS-Xiaoxi-v2-DFbrand-cbk-final.pdf, 2022

Publications

Characterization of Therapeutic mAb Charge Heterogeneity by iCIEF Coupled to Mass Spectrometry (iCIEF–MS)Global characterization of modifications to the charge isomers of IgG antibody
Johannes Schlecht, Bernd Moritz, Steffen Kiessig, Christian Neusüß
September23, 2022, https://orcid.org/0000-0001-9071-5904

Global characterization of modifications to the charge isomers of IgG antibody
Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao,Xiaohong Qian, Tao Zhu, Wantao Ying
Journal of Pharmaceutical Analysis, 12 (2022) 156-163

High-Efficiency Preparative Imaged Capillary Isoelectric Focusing (iCIEF) and iCIEF-MS Protein Charge Variant Characterisation
Tong Chen, Teresa Kwok, Anne Rose Esmin, Victor Li, Gerard Rozing,Tiemin Huang
Chromatography, May / June 2021

High-Throughput Precision with On-Board Mixing for Imaged Capillary Isoelectric Focusing (iCIEF)
Katelyn Cousteils, Jennifer Hu, Victor Li, Tiemin Huang
Chromatography, February / March 2019

Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve
Faculty of Chemistry, Aalen University, Aalen, Germany
Electrophoresis, 2018, DOI 10.1002/elps.201800013

Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging
Junjian Fang*, Jiexin Liu*, Haijing Li, Jianhua Cheng, Shengming Wu, and Fangting Dong
INSTRUMENTATION SCIENCE AND TECHNOLOGY, 2018, https://doi.org/10.1080/10739149.2017.1405821

Others

The Expanding Hyphenation of LC Techniques to MS for Intact Mass Characterization of Biopharmaceuticals During Development – Lessons Learned and The Road Ahead
Dan Bach Kristensen, Symphogen
17th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry, September 2021