Preparative iCIEF System
In response to the tremendous and urgent needs for the identification of protein charge variants, Advanced Electrophoresis Solutions Ltd. has developed the CEInfinite Automated Preparative iCIEF system.
Why choose CEInfinite?
Fast method integration and adoption
Minimum requirement of additional R&D derived from existing iCIEF platform method.
High Reproducibility and Efficiency
A fully automated system allows ~100 µg collections per day.
Capillary Diameter Transfer Technology
Our proprietary Capillary Diameter Transfer Technology (CDTT*) allows the high-resolution preparation of charge variant peaks. Once the proteins are focused, the mobilization process begins and the protein isomers are pushed out of transfer capillary. This is visualized in real time on-screen via the Whole Column Image Detection (WCID) of iCIEF, which is not presented in the single-point detection CIEF.
During mobilization, the electric field is still applied to the separation capillary and the CDTT maintains the separation resolution of the sample into the narrower transfer capillary for high purity fractions of low pI different isomers.
*US Patent 10935519
4mg/ml NISTmAb in mobilization with overall resolution of protein preserved.
|Detection mode||Whole column, sCMOS imaging technology|
|Sample capacity||84+3 vial tray, 96 well plate|
|Sample tray temperature||4 - 40 °C|
|Syringe size||250 µL|
|Detection dynamic range||250 (0.004 – 1.0 AU, 280nm)|
|High voltage range||0 – 3000 volts (continuously adjustable)|
|Sample throughput||Up to 12 injections per hour|
|Working temperature||15 - 35 °C|
|Humidity||20 - 80% RH|
|Electrical requirement||100/240 VAC, 50 - 60 Hz|
|Exposure time||0.02 – 99.9 ms|
|ADC maximum||16386 AU|
|Operation mode||Manual or automatic|
|Detector noise||Less than 0.001 AU, 280 nm|
|Separation pH range||2.1 – 11.1|